产品名称
Mouse Granzyme B/GZMB ELISA Kit
指标别名
CCPI; CGL 1; CGL1; CSP B; CSPB; CTLA1; CTSGL1; Endogenous granzyme B; Granzyme B; GZMB; HLP; SECT
免疫原
Expression system for standard: NS0; Immunogen sequence: I21-S247
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Granzyme B/GZMB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1115)
背景资料
Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1115-CAP | 96-well plate precoated with anti- mouse Granzyme B antibody | 1 |
EK1115-ST | Lyophilized recombinant mouse Granzyme B standard | 10ng/tube×2 |
EK1115-DA | Biotinylated anti- mouse Granzyme B antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 31 | 151 | 486 | 31 | 149 | 483 |
Standard deviation | 1.39 | 11.77 | 25.75 | 1.89 | 14 | 29.94 |
CV(%) | 4.5% | 7.8% | 5.3% | 6.1% | 9.4% | 6.2% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.018 | 0.059 | 0.109 | 0.199 | 0.385 | 0.686 | 1.231 | 2.036 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)