产品名称
Human CD138/Syndecan-1/SDC1 ELISA Kit
指标别名
CD138; CD138/Syndecan-1; SDC; SDC1; SYND1; syndecan; syndecan 1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
文献引用格式
Human CD138/Syndecan-1/SDC1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1339)
背景资料
Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1339-CAP | 96-well plate precoated with anti- human Syndecan-1 antibody | 1 |
EK1339-ST | Lyophilized recombinant human Syndecan-1 standard | 20ng/tube×2 |
EK1339-DA | Biotinylated anti- human Syndecan-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 632 | 2595 | 8396 | 676 | 2617 | 8785 |
Standard deviation | 25.91 | 171.27 | 545.74 | 31.09 | 196.27 | 588.59 |
CV(%) | 4.1 | 6.6 | 6.5 | 4.6 | 7.5 | 6.7 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.011 | 0.060 | 0.099 | 0.196 | 0.393 | 0.722 | 1.427 | 2.177 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum