产品名称
Mouse CD138/Syndecan-1/SDC1 ELISA Kit
指标别名
CD138; CD138/Syndecan-1; SDC; SDC1; SYND1; syndecan; syndecan 1
免疫原
Expression system for standard: NS0; Immunogen sequence: Q18-E252
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse CD138/Syndecan-1/SDC1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1554)
背景资料
Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1554-CAP | 96-well plate precoated with anti- mouse Syndecan-1 antibody | 1 |
EK1554-ST | Lyophilized recombinant mouse Syndecan-1 standard | 10ng/tube×2 |
EK1554-DA | Biotinylated anti- mouse Syndecan-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 326 | 1681 | 5402 | 348 | 1654 | 5887 |
Standard deviation | 25.1 | 122.71 | 383.54 | 29.92 | 129.01 | 476.84 |
CV(%) | 7.7% | 7.3% | 7.1% | 8.6% | 7.8% | 8.1% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.002 | 0.083 | 0.147 | 0.314 | 0.636 | 1.159 | 1.802 | 2.258 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)