产品名称
Chinese Hamster GDF5 ELISA Kit
指标别名
growth differentiation factor 5; BDA1C; BMP-14; BMP14; CDMP1; DUPANS; LAP-4; LAP4; OS5; SYM1B; SYNS2; GDF-5; GDF5
免疫原
Expression system for standard: E.coli; Immunogen sequence: A376-R495
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
growth differentiation factor 5
文献引用格式
Chinese Hamster GDF5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1504-HA)
背景资料
Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.
试剂盒内容
Catalog number | Description | Quantity |
EK1504-HA-CAP | Anti-Hamster GDF5 Pre-coated 96-well strip microplate | 1 |
EK1504-HA-ST | Hamster GDF5 Standard | 10ng/tube×2 |
EK1504-HA-DA | Hamster GDF5 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 196 | 753 | 1345 | 221 | 834 | 1416 |
Standard deviation | 10.97 | 51.2 | 99.53 | 16.57 | 69.22 | 121.77 |
CV (%) | 6.8 | 6.8 | 7.4 | 7.5 | 8.3 | 8.6 |
典型数据示例
Concentration(pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
O.D. | 0.055 | 0.106 | 0.161 | 0.258 | 0.448 | 0.797 | 1.365 | 2.007 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)