产品名称
Mouse GDF5 ELISA Kit
指标别名
growth differentiation factor 5; BDA1C; BMP-14; BMP14; CDMP1; DUPANS; LAP-4; LAP4; OS5; SYM1B; SYNS2; GDF-5; GDF5
免疫原
Expression system for standard: E.coli; Immunogen sequence: A376-R495
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Growth/differentiation factor 5
文献引用格式
Mouse GDF5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1505)
背景资料
Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1505-CAP | 96-well plate precoated with anti- mouse GDF5 antibody | 1 |
EK1505-ST | Lyophilized recombinant mouse GDF5 standard | 10ng/tube×2 |
EK1505-DA | Biotinylated anti- mouse GDF5 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 69 | 222 | 1053 | 62 | 217 | 1005 |
Standard deviation | 4.34 | 15.98 | 63.18 | 4.89 | 17.14 | 62.31 |
CV(%) | 6.3% | 7.2% | 6% | 7.9% | 7.9% | 6.2% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.024 | 0.147 | 0.248 | 0.431 | 0.857 | 1.390 | 1.932 | 2.243 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)