产品名称
Human BMP9/GDF2 ELISA Kit
指标别名
BMP 9; BMP9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
growth differentiation factor 2
文献引用格式
Human BMP9/GDF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1560)
背景资料
Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1560-CAP | 96-well plate precoated with anti- human BMP-9 antibody | 1 |
| EK1560-ST | Lyophilized recombinant human BMP-9 standard | 10ng/tube×2 |
| EK1560-DA | Biotinylated anti- human BMP-9 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 186 | 405 | 34 | 178 | 376 |
| Standard deviation | 2.06 | 9.3 | 19.44 | 2.48 | 11.92 | 24.44 |
| CV(%) | 5.9 | 5 | 4.8 | 7.3 | 6.7 | 6.5 |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.038 | 0.157 | 0.215 | 0.329 | 0.564 | 0.961 | 1.660 | 2.324 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum Plasma (heparin, EDTA) and Urine