Mouse BMP9/GDF2 ELISA Kit

96T/2480

BMP 9; BMP9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2

Expression system for standard: CHO; Immunogen sequence: S319-R428

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

15.6pg/ml-1000pg/ml

Gdf2

growth differentiation factor 2

Q9WV56

Mouse BMP9/GDF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1610)

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Catalog number	Description	Quantity
EK1610-CAP	96-well plate precoated with anti- mouse BMP-9 antibody	1
EK1610-ST	Lyophilized recombinant mouse BMP-9 standard	10ng/tube×2
EK1610-DA	Biotinylated anti- mouse BMP-9 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	39	220	419	41	226	402
Standard deviation	1.71	11.22	31.42	2.05	14.46	30.95
CV(%)	4.4%	5.1%	7.5%	5%	6.4%	7.7%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.008	0.133	0.221	0.388	0.688	1.140	1.757	2.229

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h