指标别名
CV2; CV-2; CRIM3; BMPER; KIAA1965
免疫原
Expression system for standard: NS0; Immunogen sequence: V34-R685
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
BMP-binding endothelial regulator
文献引用格式
Rat BMPER ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2120)
背景资料
This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.
试剂盒内容
Catalog number | Description | Quantity |
EK2120-CAP | Anti-Rat Bmper Pre-coated 96-well strip microplate | 1 |
EK2120-ST | Rat Bmper Standard | 10ng/tube×2 |
EK2120-DA | Rat Bmper Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 226 | 813 | 1749 | 235 | 854 | 1806 |
Standard deviation | 10.62 | 45.53 | 101.44 | 15.04 | 62.34 | 140.87 |
CV(%) | 4.7% | 5.6% | 5.8% | 6.4% | 7.3% | 7.8% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.068 | 0.172 | 0.254 | 0.376 | 0.612 | 0.922 | 1.624 | 2.289 |
特异性
Natural and recombinant rat BMPER
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)