Anti-LOX Antibody (Clone#OTI5C4)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

M00575-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00575-2)
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Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX .
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产品简介>

产品名称

Anti-LOX Antibody (Clone#OTI5C4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

LOX; lysyl oxidase; Protein lysine 6 oxidase

产品类型

Monoclonal

检验物种

human, mouse, rat, dog

应用范围

WB, IHC, ICC/IF, FCM

基因名称

LOX

克隆号

OTI5C4

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.

计算分子量

47 kDa

实际分子量

46.8 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot ID

P28300

文献引用格式

LOX Antibody (Clone#OTI5C4) (Boster Biological Technology, Wuhan, China. Catalog#M00575-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

lysyl oxidase

蛋白名全称

Protein-lysine 6-oxidase

实验方案>

实验步骤

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