Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |
Flow Cytometry (FCM): | 1:20 |
Western blot analysis of anti-LOX antibody (BM5132). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LOX antigen affinity purified monoclonal antibody (BM5132) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
IHC analysis of LOX using anti-LOX antibody (BM5132) .
LOX was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-LOX Antibody (BM5132) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of LOX using anti-LOX antibody (BM5132) .
LOX was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-LOX Antibody (BM5132) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of LOX using anti-LOX antibody (BM5132) .
LOX was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-LOX Antibody (BM5132) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of anti-LOX antibody (BM5132). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LOX antigen affinity purified monoclonal antibody (BM5132) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
IHC analysis of LOX using anti-LOX antibody (BM5132) .
LOX was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-LOX Antibody (BM5132) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of LOX using anti-LOX antibody (BM5132) .
LOX was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-LOX Antibody (BM5132) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of LOX using anti-LOX antibody (BM5132) .
LOX was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-LOX Antibody (BM5132) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.