Anti-SIRT6 Antibody (Clone#OTI1G3)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF

M00611-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT6(Cat# M00611-3).
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Anti-SIRT6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT6 .
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产品简介>

产品名称

Anti-SIRT6 Antibody (Clone#OTI1G3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

SIR2 like protein 6; SIR2L6; SIRT6

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

SIRT6

克隆号

OTI1G3

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 88-352 of human SIRT6(NP_057623) produced in E.coli.

计算分子量

39 kDa

实际分子量

39-42 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.

Uniprot ID

Q8N6T7

文献引用格式

SIRT6 Antibody (Clone#OTI1G3) (Boster Biological Technology, Wuhan, China. Catalog#M00611-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

sirtuin 6

蛋白名全称

NAD-dependent protein deacetylase sirtuin-6

实验方案>

实验步骤

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