| Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:150 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| Flow Cytometry (FCM): | 1:100 |

Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 3. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 4. Living HEK293T cells transfected with either plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 7. Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY PECAM1 using anti-PECAM1 mouse monoclonal antibody. Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control (merged, lower right) (1:100).

Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 3. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 4. Living HEK293T cells transfected with either plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

Figure 7. Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY PECAM1 using anti-PECAM1 mouse monoclonal antibody. Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control (merged, lower right) (1:100).

Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.







