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Anti-CD31/PECAM1 Antibody (Clone#OTI1H4)

Mouse monoclonal antibody

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筛选器: All IHC FCM ICC/IF WB

M01513-5

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

    all(8)
  • Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

    all(8)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

    all(8)
  • Figure 4. Living HEK293T cells transfected with either plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

    all(8)
  • Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

    all(8)
  • Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01513-5)

    all(8)
  • Figure 7. Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY PECAM1 using anti-PECAM1 mouse monoclonal antibody. Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control (merged, lower right) (1:100).

    all(8)
  • Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

    all(8)

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产品名称
Anti-CD31/PECAM1 Antibody (Clone#OTI1H4)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
CD31; EndoCAM; GPIIA; PECA1; PECAM 1; PECAM1; VEC marker
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, FCM
基因名称
PECAM1
克隆号
OTI1H4
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protien of human PECAM1(NP_000433) produced in HEK293T cell.
计算分子量
83 kDa
实际分子量
82.4 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq]
Uniprot ID
P16284  
文献引用格式
CD31/PECAM1 Antibody (Clone#OTI1H4) (Boster Biological Technology, Wuhan, China. Catalog#M01513-5)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
platelet/endothelial cell adhesion molecule 1
蛋白名全称
Platelet endothelial cell adhesion molecule
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
Flow Cytometry (FCM):1:100

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    Anti-CD31/PECAM1 Antibody (Clone#OTI1H4)

    筛选器: All IHC FCM ICC/IF WB

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