Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.
all(4)
Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M01705-5)
all(4) 
Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
all(4) 
Figure 4. Anti-IDO1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1 .
all(4) | Western blot (WB): | 1:200~2000 |
| Immunohistochemistry (IHC): | 1:150 |
| Immunofluorescence (IF): | 1:100 |
Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.
Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M01705-5)
Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 4. Anti-IDO1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1 .
Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO1.
Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M01705-5)
Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 4. Anti-IDO1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDO1 .
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