产品名称
Anti-Hu CD177 Purified Antibody (Clone#MEM-166)
指标别称
NB1; PRV1; HNA2A; CD177; UNQ595/PRO1181; HNA-2a; NB1 GP; PRV-1
免疫原
Human granulocytes. The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
成分
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方式
Purified by protein-A affinity chromatography.
保存条件
Store at 2-8°C. Do not freeze.
背景资料
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
文献引用格式
Hu CD177 Purified Antibody (Clone#MEM-166) (Boster Biological Technology, Wuhan, China. Catalog#M03958)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Flow cytometry: 1 µg/ml.
Western blotting: Non-reducing conditions.