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Anti-CD10/MME Antibody (Clone#OTI3D11)

Mouse monoclonal antibody

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筛选器: All WB IHC FCM

M04065-3

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MME (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (Cat# M04065-3)(1:500).

    all(12)
  • Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-MME monoclonal antibody (1:500).

    all(12)
  • Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

    all(12)
  • Flow cytometric analysis of living 293T cells transfected with MME overexpression plasmid , Red)/empty vector (Blue) using anti-MME antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

    all(12)

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产品简介>

产品名称
Anti-CD10/MME Antibody (Clone#OTI3D11)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Atriopeptidase; CALLA; CD10; Enkephalinase; EPN; membrane metallo endopeptidase; MME; MME; CD10; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, FCM
基因名称
MME
克隆号
OTI3D11
宿主
Mouse
抗体亚型
IgG2a
免疫原
Full length human recombinant protein of human MME(NP_009218) produced in HEK293T cell.
计算分子量
86 kDa
实际分子量
85.3 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq]
Uniprot ID
P08473  
文献引用格式
CD10/MME Antibody (Clone#OTI3D11) (Boster Biological Technology, Wuhan, China. Catalog#M04065-3)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Neprilysin
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150
Flow cytometry (FCM):1:100

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    Anti-CD10/MME Antibody (Clone#OTI3D11)

    筛选器: All WB IHC FCM

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