Anti-AMPK Beta 2/PRKAB2 Antibody (Clone#6G1)

筛选器： All FCM WB

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  Figure 1. Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody (M05077).Overlay histogram showing PC-3 cells stained with M05077 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (M05077, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Figure 2. Western blot analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (M05077). Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysate,
  Lane 2: human placenta tissue lysate,
  Lane 3: human 293T whole cell lysate,
  Lane 4: human A549 whole cell lysate,
  Lane 5: human A375 whole cell lysate,
  Lane 6: human A431 whole cell lysate,
  Lane 7: human U20S whole cell lysate,
  Lane 8: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AMPK beta 2 antigen affinity purified monoclonal antibody (Catalog # M05077) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.