Anti-Beta Tubulin/TUBB Antibody (Clone#17T13)

Mouse monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF

M05613-5

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-Beta Tubulin/TUBB antibody (M05613-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Beta Tubulin/TUBB antigen affinity purified monoclonal antibody (M05613-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Tubulin/TUBB at approximately 50 kDa. The expected band size for Beta Tubulin/TUBB is at 50 kDa.
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Figure 2.Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using beta Tubulin Mouse Monoclonal Antibody.
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Figure 3.Immunofluorescent analysis of Hela cells, using beta Tubulin mAb.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Beta Tubulin/TUBB Antibody (Clone#17T13)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

beta tubulin; B-tubulin; tbb5; TUBB; tubulin; β-tubulin

产品类型

Monoclonal

检验物种

human, mouse, rat, chicken, hamster

应用范围

WB, IHC, ICC/IF

基因名称

TUBB

克隆号

17T13

抗体来源

Mouse

抗体亚型

IgG1

免疫原

A synthesized peptide derived from human beta Tubulin

计算分子量

50 kDa

实际分子量

50 kDa

成分

Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.5mg/ml BSA.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia,complex,with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1,6,7,8,9,and 13.

Uniprot ID

P07437

文献引用格式

Beta Tubulin/TUBB Antibody (Clone#17T13) (Boster Biological Technology, Wuhan, China. Catalog#M05613-5)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

实验方案>

实验步骤

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