Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 tissue lysates,Lane 2: monkey COS-7 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Hela whole cell lysates,Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.
Figure 2. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat liver whole cell lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain whole cell lysates,Lane 5: mouse NIH3T3, whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.
Figure 5. Flow cytometry analysis of U937 cell(1:100) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).
Figure 3. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 4. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 tissue lysates,Lane 2: monkey COS-7 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Hela whole cell lysates,Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.
Figure 2. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat liver whole cell lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain whole cell lysates,Lane 5: mouse NIH3T3, whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.
Figure 5. Flow cytometry analysis of U937 cell(1:100) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).
Figure 3. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 4. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).