Anti-TIE2/TEK Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0179

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TIE2/TEK using anti-TIE2/TEK antibody (PB0179).
Lane 1: recombinant human TIE2 protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIE2/TEK antigen affinity purified polyclonal antibody (PB0179) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIE2/TEK at approximately 47 kDa.
all(2)
Western blot analysis of TIE2/TEK using anti-TIE2/TEK antibody (PB0179). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIE2/TEK antigen affinity purified polyclonal antibody (PB0179) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIE2/TEK at approximately 160 kDa. The expected band size for TIE2/TEK is at 126 kDa.
all(2)

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产品名称

Anti-TIE2/TEK Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Angiopoietin 1 receptor; CD202B; hTIE2; p140 TEK; TEK; TIE 2; Tie2; VMCM; VMCM1

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

TEK

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human TIE2 recombinant protein (Position: Q641-I830). Human TIE2 shares 91% amino acid (aa) sequence identity with mouse TIE2.

计算分子量

126 kDa

实际分子量

160 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

Uniprot ID

Q02763

文献引用格式

TIE2/TEK Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0179)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

TEK tyrosine kinase, endothelial

蛋白名全称

Angiopoietin-1 receptor

实验方案>

实验步骤

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