Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of Anti-TEK antibody (PB0179). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Recombinant human TIE2 protein 0.5ng,Use rabbit Anti-TEK 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TEK at approximately 47KD. The expected band size for TEK is at 47KD.
Figure 2. Western blot analysis of Anti-TEK antibody (PB0179). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: MCF-7 whole cell lysates,Use rabbit Anti-TEK 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TEK at approximately 125KD. The expected band size for TEK is at 125KD.
Figure 1. Western blot analysis of Anti-TEK antibody (PB0179). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Recombinant human TIE2 protein 0.5ng,Use rabbit Anti-TEK 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TEK at approximately 47KD. The expected band size for TEK is at 47KD.
Figure 2. Western blot analysis of Anti-TEK antibody (PB0179). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: MCF-7 whole cell lysates,Use rabbit Anti-TEK 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TEK at approximately 125KD. The expected band size for TEK is at 125KD.