Anti-NMDAR2B/GRIN2B Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0414

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NMDAR2B/GRIN2B using anti-NMDAR2B/GRIN2B antibody (PB0414). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NMDAR2B/GRIN2B antigen affinity purified polyclonal antibody (PB0414) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NMDAR2B/GRIN2B at approximately 180-190 kDa. The expected band size for NMDAR2B/GRIN2B is at 166 kDa.
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产品名称

Anti-NMDAR2B/GRIN2B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GluN2B; GRIN2B; hNR3; NMDAR2B; NMDAR2B/GRIN2B; NR2B; NR3

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

基因名称

GRIN2B

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human NMDAR2B recombinant protein (Position: N1076-D1332). Human NMDAR2B shares 97.7% and 97.3% amino acid (aa) sequence identity with mouse and rat NMDAR2B, respectively.

计算分子量

180-190 kDa

实际分子量

166 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The N-methyl-D-aspartate receptor 2B (NMDAR2B), also names as GRIN2B. The sequence of the predicted 1,484-amino acid human protein is 98% and 96% identical to the sequences of the rat and mouse Nmdar2b proteins, respectively. Nmdar2B gene is located on mouse chromosome 6 between Rho and Ly49 centromerically and Glb telomerically. Mapping of the human NMDAR2B receptor subunit gene (GRIN2B) to chromosome 12p12 overexpression of NMDA receptor 2B (NR2B) in the forebrains of transgenic mice leads to enhanced activation of NMDA receptors, facilitating synaptic potentiation in response to stimulation at 10-100 Hz.

Uniprot ID

Q13224

文献引用格式

NMDAR2B/GRIN2B Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0414)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

glutamate receptor, ionotropic, N-methyl D-aspartate 2B

蛋白名全称

Glutamate receptor ionotropic, NMDA 2B

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