Anti-S100A8 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

PB0767

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of S100A8 using anti-S100A8 antibody (PB0767). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A8 antigen affinity purified polyclonal antibody (PB0767) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for S100A8 at approximately 10 kDa. The expected band size for S100A8 is at 10 kDa.
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IHC analysis of S100A8 using anti-S100A8 antibody (PB0767).
S100A8 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-S100A8 Antibody (PB0767) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of S100A8 using anti-S100A8 antibody (PB0767).
S100A8 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-S100A8 Antibody (PB0767) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of S100A8 using anti-S100A8 antibody (PB0767).
S100A8 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-S100A8 Antibody (PB0767) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of S100A8 using anti-S100A8 antibody (PB0767).
S100A8 was detected in an immunocytochemical section of NIH/3T3 cells. The section was incubated with rabbit anti-S100A8 Antibody (PB0767) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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IF analysis of S100A8 using anti-S100A8 antibody (PB0767).
S100A8 was detected in an immunocytochemical section of MFC cells. The section was incubated with rabbit anti-S100A8 Antibody (PB0767) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of Hepa1-6 cells using anti-S100A8 antibody (PB0767).
Overlay histogram showing Hepa1-6 cells stained with PB0767 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A8 Antibody (PB0767) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-S100A8 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

60B8AG; CAGA; Calgranulin A; Calprotectin L1L subunit; CFAG; CGLA; CP 10; Cystic fibrosis antigen; L1Ag; MA387; MIF; MRP 8; MRP8; NIF; P8; Protein S100 A8; S100A8; Urinary stone protein band A

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

S100A8

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.

计算分子量

10 kDa

实际分子量

10 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Uniprot ID

P27005

文献引用格式

S100A8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0767)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

S100 calcium binding protein A8

蛋白名全称

Protein S100-A8

实验方案>

实验步骤

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