Anti-CD138/Syndecan-1/SDC1 Antibody

筛选器： All WB ICC/IF FCM

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  Figure 1. Western blot analysis of Syndecan-1 using anti-Syndecan-1 antibody (PB0791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HT1080 Whole Cell Lysate,Lane 2: MCF-7 Whole Cell Lysate,Lane 3: 293T Whole Cell Lysate,Lane 4: HEPG2 Whole Cell Lysate,Lane 5: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1 antigen affinity purified polyclonal antibody (Catalog # PB0791) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1 at approximately 50KD. The expected band size for Syndecan-1 is at 32KD.

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  Figure 2. ICC analysis using anti-Syndecan-1 antibody (PB0791) was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).

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  Figure 3. Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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  Figure 1. Western blot analysis of Syndecan-1 using anti-Syndecan-1 antibody (PB0791). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HT1080 Whole Cell Lysate,Lane 2: MCF-7 Whole Cell Lysate,Lane 3: 293T Whole Cell Lysate,Lane 4: HEPG2 Whole Cell Lysate,Lane 5: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1 antigen affinity purified polyclonal antibody (Catalog # PB0791) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1 at approximately 50KD. The expected band size for Syndecan-1 is at 32KD.

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  Figure 2. ICC analysis using anti-Syndecan-1 antibody (PB0791) was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).

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  Figure 3. Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).