Figure 1. Western blot analysis of anti- PGK1 antibody (PB0824). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
Use rabbit anti- PGK1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PGK1 at approximately 43KD. The expected band size for PGK1 is at 45KD.
Figure 2. ICC analysis using anti- PGK1 antibody (PB0824). was detected in immersion fixed U-87 MG cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032).
all(3) 
Figure 3. Flow cytometry analysis of 293T cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(3) | Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of anti- PGK1 antibody (PB0824). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
Use rabbit anti- PGK1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PGK1 at approximately 43KD. The expected band size for PGK1 is at 45KD.
Figure 2. ICC analysis using anti- PGK1 antibody (PB0824). was detected in immersion fixed U-87 MG cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032).
Figure 3. Flow cytometry analysis of 293T cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of anti- PGK1 antibody (PB0824). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
Use rabbit anti- PGK1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PGK1 at approximately 43KD. The expected band size for PGK1 is at 45KD.
Figure 2. ICC analysis using anti- PGK1 antibody (PB0824). was detected in immersion fixed U-87 MG cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032).
Figure 3. Flow cytometry analysis of 293T cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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