Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of ISG15 expression in 22RV1 whole cell lysates (lane 1) and HELA whole cell lysates (lane 2). ISG15 at 15KD was detected using rabbit anti- ISG15 Antigen Affinity purified polyclonal antibody (Catalog # PB0999) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
ISG15 was detected in paraffin-embedded sections of human pancreatic cancer tissues using rabbit anti- ISG15 Antigen Affinity purified polyclonal antibody (Catalog # PB0999) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Western blot analysis of ISG15 expression in 22RV1 whole cell lysates (lane 1) and HELA whole cell lysates (lane 2). ISG15 at 15KD was detected using rabbit anti- ISG15 Antigen Affinity purified polyclonal antibody (Catalog # PB0999) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
ISG15 was detected in paraffin-embedded sections of human pancreatic cancer tissues using rabbit anti- ISG15 Antigen Affinity purified polyclonal antibody (Catalog # PB0999) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).