Anti-ISG15 Antibody

筛选器： All WB IHC

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  Figure 1. Western blot analysis of ISG15 using anti-ISG15 antibody (PB1000). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ISG15 antigen affinity purified polyclonal antibody (PB1000) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ISG15 at approximately 18 kDa. The expected band size for ISG15 is at 18 kDa.

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  Figure 2. IHC analysis of ISG15/Ucrp using anti- ISG15/Ucrp antibody (PB1000).ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- ISG15/Ucrp Antibody (PB1000) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  Figure 1. Western blot analysis of ISG15 using anti-ISG15 antibody (PB1000). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ISG15 antigen affinity purified polyclonal antibody (PB1000) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ISG15 at approximately 18 kDa. The expected band size for ISG15 is at 18 kDa.

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  Figure 2. IHC analysis of ISG15/Ucrp using anti- ISG15/Ucrp antibody (PB1000).ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- ISG15/Ucrp Antibody (PB1000) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.