规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
AQP11; AQPX1; PSEC0027; Aquaporin-11; AQP-11
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human AQP11 different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
AQP11 has a unique asparagine-proline-alanine (NPA) box distinct from those of other AQPs, suggesting a different pore structure and function. Using Northern blot analysis, they detected highest expression of mouse Aqp11 in testis, followed by liver and kidney. Expression was much weaker in heart, brain, and muscle. Western blot analysis of mouse kidney membrane fractions detected Aqp11 at an apparent molecular mass of 26 kD, lower than the calculated molecular mass of 30 kD. Immunohistochemical analysis localized Aqp11 to mouse renal proximal tubule cells, where it showed a perinuclear distribution. Fluorescence-tagged Aqp11 localized with an endoplasmic reticulum marker.
文献引用格式
AQP11 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1098)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB): | 1:500-2000 |