Figure 1. Western blot analysis of anti- TNF alpha/Tnf antibody (A00002-5).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates.Use rabbit anti- TNF alpha/Tnf 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TNF alpha/Tnf at approximately 26KD. The expected band size for TNF alpha/Tnf is at 26KD.
all(4)
Figure 2. ICC analysis using anti- TNF alpha/Tnf antibody (A00002-5). was detected in immersion fixed mouse HEPA1-6 cell line . Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
all(4) 
Figure 3. Flow cytometry analysis of RAW264.7 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(4) 
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- TNF alpha/Tnf antibody (A00002-5).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates.Use rabbit anti- TNF alpha/Tnf 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TNF alpha/Tnf at approximately 26KD. The expected band size for TNF alpha/Tnf is at 26KD.
Figure 2. ICC analysis using anti- TNF alpha/Tnf antibody (A00002-5). was detected in immersion fixed mouse HEPA1-6 cell line . Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of RAW264.7 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- TNF alpha/Tnf antibody (A00002-5).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates.Use rabbit anti- TNF alpha/Tnf 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TNF alpha/Tnf at approximately 26KD. The expected band size for TNF alpha/Tnf is at 26KD.
Figure 2. ICC analysis using anti- TNF alpha/Tnf antibody (A00002-5). was detected in immersion fixed mouse HEPA1-6 cell line . Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of RAW264.7 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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