Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U-937 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse spleen tissue lysates.
Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.
Figure 2. IHC analysis using anti- TNFA/TNF Alpha antibody (BA0131). detected in paraffin-embedded section of human B lymphocytic tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
all(3) 
Figure 3. Flow cytometry analysis of Caco-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(3) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U-937 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse spleen tissue lysates.
Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.
Figure 2. IHC analysis using anti- TNFA/TNF Alpha antibody (BA0131). detected in paraffin-embedded section of human B lymphocytic tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of Caco-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U-937 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse spleen tissue lysates.
Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.
Figure 2. IHC analysis using anti- TNFA/TNF Alpha antibody (BA0131). detected in paraffin-embedded section of human B lymphocytic tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of Caco-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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