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Anti-TNF Alpha/TNFA Antibody (Clone#OTI1D9)

Mouse monoclonal antibody

筛选器: All IHC WB

M00002-4

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00002-4)

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  • Figure 2. Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00002-4)

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  • Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNF.

    all(3)

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产品名称
Anti-TNF Alpha/TNFA Antibody (Clone#OTI1D9)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC
基因名称
TNF
克隆号
OTI1D9
抗体来源
Mouse
抗体亚型
IgG2a
免疫原
Full length human recombinant protein of human TNF(NP_000585) produced in HEK293T cell.
计算分子量
26 kDa
实际分子量
26 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
TNF alpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Uniprot ID
P06804  
文献引用格式
TNF Alpha/TNFA Antibody (Clone#OTI1D9) (Boster Biological Technology, Wuhan, China. Catalog#M00002-4)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
tumor necrosis factor
蛋白名全称
Tumor necrosis factor
推荐稀释比
Western blot (WB):1:4000
Immunohistochemistry (IHC):1:150

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    Anti-TNF Alpha/TNFA Antibody (Clone#OTI1D9)

    筛选器: All IHC WB

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