Mouse monoclonal antibody
Western blot (WB): | 1:4000 |
Immunohistochemistry (IHC): | 1:150 |
Figure 1. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00002-4)
Figure 2. Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00002-4)
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNF.
Figure 1. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00002-4)
Figure 2. Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00002-4)
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNF.