Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (A00040-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate,Lane 2: mouse thymus tissue lysate,Lane 3: human MCF-7 whole Cell lysate,Lane 4: human 22RV1 whole Cell lysate. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bcl-2 at approximately 29KD. The expected band size for Bcl-2 is at 26KD.
all(3)
Figure 2. IHC analysis of Bcl-2 using anti- Bcl-2 antibody (A00040-1).
Bcl-2 was detected in paraffin-embedded section of huamn intestinal tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow Cytometry analysis of U937 cells using anti- Bcl-2 antibody (A00040-1).
Overlay histogram showing U937 cells stained with A00040-1 (Blue line).. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (A00040-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate,Lane 2: mouse thymus tissue lysate,Lane 3: human MCF-7 whole Cell lysate,Lane 4: human 22RV1 whole Cell lysate. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bcl-2 at approximately 29KD. The expected band size for Bcl-2 is at 26KD.
Figure 2. IHC analysis of Bcl-2 using anti- Bcl-2 antibody (A00040-1).
Bcl-2 was detected in paraffin-embedded section of huamn intestinal tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow Cytometry analysis of U937 cells using anti- Bcl-2 antibody (A00040-1).
Overlay histogram showing U937 cells stained with A00040-1 (Blue line).. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (A00040-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate,Lane 2: mouse thymus tissue lysate,Lane 3: human MCF-7 whole Cell lysate,Lane 4: human 22RV1 whole Cell lysate. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Bcl-2 at approximately 29KD. The expected band size for Bcl-2 is at 26KD.
Figure 2. IHC analysis of Bcl-2 using anti- Bcl-2 antibody (A00040-1).
Bcl-2 was detected in paraffin-embedded section of huamn intestinal tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow Cytometry analysis of U937 cells using anti- Bcl-2 antibody (A00040-1).
Overlay histogram showing U937 cells stained with A00040-1 (Blue line).. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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