Anti-BCL2 Antibody (Clone#OTI2E5)

Mouse monoclonal antibody

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筛选器： All IHC WB

MA00040

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00040)
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Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-BCL2 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA00040)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
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Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-BCL2 monoclonal antibody (1:500).
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产品简介>

产品名称

Anti-BCL2 Antibody (Clone#OTI2E5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptosis regulator Bcl 2; B cell CLL/lymphoma 2; BCL2; BCL-2; Oncogene B cell leukemia 2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

BCL2

克隆号

OTI2E5

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human BCL2 (NP_000624) produced in E.coli.

计算分子量

26 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Uniprot ID

P10415

文献引用格式

BCL2 Antibody (Clone#OTI2E5) (Boster Biological Technology, Wuhan, China. Catalog#MA00040)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

B-cell CLL/lymphoma 2

蛋白名全称

Apoptosis regulator Bcl-2

实验方案>

实验步骤

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