Figure 1. Western blot analysis of anti- BCL2 antibody (A00040-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
Use rabbit anti- BCL2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BCL2 at approximately 26KD. The expected band size for BCL2 is at 26KD.
Figure 2. ICC analysis using anti- BCL2 antibody (A00040-2) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed U2OS cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).
all(4) 
Figure 3. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(4) 
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- BCL2 antibody (A00040-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
Use rabbit anti- BCL2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BCL2 at approximately 26KD. The expected band size for BCL2 is at 26KD.
Figure 2. ICC analysis using anti- BCL2 antibody (A00040-2) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed U2OS cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).
Figure 3. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- BCL2 antibody (A00040-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
Use rabbit anti- BCL2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BCL2 at approximately 26KD. The expected band size for BCL2 is at 26KD.
Figure 2. ICC analysis using anti- BCL2 antibody (A00040-2) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed U2OS cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).
Figure 3. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
