Anti-VEGF/VEGFA Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB ELISA

A00045-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of anti-Vegfa antibody (A00045-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: mouse thymus tissue lysates.Use rabbit anti- Vegfa 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for Vegfa at approximately 27KD. The expected band size for Vegfa is at 25KD.
all(2)
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-VEGF/VEGFA Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB,ELISA

基因名称

Vegfa

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse VEGF/Vegfa recombinant protein (Position: E38-R214).

计算分子量

25 kDa

实际分子量

27 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Vascular endothelial growth factor A (VEGF-A) is a protein that in humans is encoded by the VEGFA gene. It is mapped to 17 C; 17 22.79 cM. This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein,which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells,and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome,also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism,and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame,which is located within an internal ribosome entry site.

Uniprot ID

Q00731

RRID

AB_3080911

文献引用格式

VEGF/VEGFA Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00045-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

vascular endothelial growth factor A

蛋白名全称

Vascular endothelial growth factor A

实验方案>

实验步骤

引用文献(0)>

清除