Anti-VEGF/VEGFA Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

PB9071

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (PB9071).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysate,Lane 2: rat lver tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse lung tissue lysate,Lane 5: mouse thymus tissue lysate,Lane 6: HEPA1-6 whole cell lysate,Lane 7: human placenta tissue lysate,Lane 8: A431 whole cell lysate,Use rabbit anti- VEGF 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for VEGF at approximately 27KD. The expected band size for VEGF is at 27KD.
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Figure 2. IHC analysis using anti- VEGF antibody (PB9071). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis using anti- VEGF antibody (PB9071). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis using anti- VEGF antibody (PB9071). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 5. Flow cytometry analysis of SiHa cell HELA(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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产品简介>

产品名称

Anti-VEGF/VEGFA Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

VEGFA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.

计算分子量

27 kDa

实际分子量

27 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.

Uniprot ID

P15692

RRID

AB_3081956

文献引用格式

VEGF/VEGFA Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9071)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

vascular endothelial growth factor A

蛋白名全称

Vascular endothelial growth factor A

实验方案>

实验步骤

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