Figure 1. Western blot analysis of anti- ABCB1 antibody (A00049-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates,Lane 2: mouse liver tissue lysates. Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 130-180KD. The expected band size for ABCB1 is at 141KD.
all(4)
Figure 3. IHC analysis using anti-ABCB1 antibody (A00049-3).detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(4) 
Figure 2. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(4) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- ABCB1 antibody (A00049-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates,Lane 2: mouse liver tissue lysates. Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 130-180KD. The expected band size for ABCB1 is at 141KD.
Figure 3. IHC analysis using anti-ABCB1 antibody (A00049-3).detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- ABCB1 antibody (A00049-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates,Lane 2: mouse liver tissue lysates. Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 130-180KD. The expected band size for ABCB1 is at 141KD.
Figure 3. IHC analysis using anti-ABCB1 antibody (A00049-3).detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. Flow cytometry analysis of U2OS cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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