Figure 1. Western blot analysis of anti- P Glycoprotein/ABCB1 Antibody (A00049-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Caco-2 whole cell lysates.
Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 141KD. The expected band size for ABCB1 is at 141KD.
Figure 3. Flow cytometry analysis of 293T cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(3) 
Figure 2. ICC analysis using anti- P Glycoprotein/ABCB1 Antibody (A00049-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
all(3) | Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of anti- P Glycoprotein/ABCB1 Antibody (A00049-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Caco-2 whole cell lysates.
Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 141KD. The expected band size for ABCB1 is at 141KD.
Figure 3. Flow cytometry analysis of 293T cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 2. ICC analysis using anti- P Glycoprotein/ABCB1 Antibody (A00049-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of anti- P Glycoprotein/ABCB1 Antibody (A00049-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Caco-2 whole cell lysates.
Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 141KD. The expected band size for ABCB1 is at 141KD.
Figure 3. Flow cytometry analysis of 293T cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 2. ICC analysis using anti- P Glycoprotein/ABCB1 Antibody (A00049-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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