| Western blot (WB): | 1:500-2000 |

Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates,Lane 2: HEPA whole cell lysates.probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (EK1002) A specific band was detected for CD11b at approximately 128KD. The expected band size for CD11b is at 127KD.

Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates,Lane 2: HEPA whole cell lysates.probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (EK1002) A specific band was detected for CD11b at approximately 128KD. The expected band size for CD11b is at 127KD.
