Anti-CD11B/Integrin Alpha M/ITGAM Antibody

Rabbit polyclonal antibody

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筛选器： All WB FCM ELISA

A00144-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
Probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD.
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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-CD11b antibody (A00144-1).Overlay histogram showing HL-60 cells stained with A00144-1 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品名称

Anti-CD11B/Integrin Alpha M/ITGAM Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

CD11B; CR 3 alpha chain; CR3A; Integrin alpha M; ITGAM; MAC 1; MAC1A; MO1A; Neutrophil adherence receptor; SLEB6

产品类型

Polyclonal

检验物种

human,mouse,rat

应用范围

WB, FCM, ELISA

基因名称

ITGAM

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human CD11b recombinant protein (Position: N129-E336).

计算分子量

127 kDa

实际分子量

170 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Uniprot ID

P11215

RRID

AB_3080973

文献引用格式

CD11B/Integrin Alpha M/ITGAM Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00144-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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