Western blot analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Emmprin/CD147/BSG antigen affinity purified polyclonal antibody (A00248-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Emmprin/CD147/BSG at approximately 35-60 kDa. The expected band size for Emmprin/CD147/BSG is at 42 kDa.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- CD147 antibody (A00248-1). detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(11) 
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(11) 
Flow cytometry analysis of Hela cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(11) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Emmprin/CD147/BSG antigen affinity purified polyclonal antibody (A00248-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Emmprin/CD147/BSG at approximately 35-60 kDa. The expected band size for Emmprin/CD147/BSG is at 42 kDa.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- CD147 antibody (A00248-1). detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Flow cytometry analysis of Hela cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Western blot analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Emmprin/CD147/BSG antigen affinity purified polyclonal antibody (A00248-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Emmprin/CD147/BSG at approximately 35-60 kDa. The expected band size for Emmprin/CD147/BSG is at 42 kDa.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- CD147 antibody (A00248-1). detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
IF analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-1).
Emmprin/CD147/BSG was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Flow cytometry analysis of Hela cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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