Anti-Emmprin/CD147/BSG Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM ELISA

A00248-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Emmprin/CD147/BSG antigen affinity purified polyclonal antibody (A00248-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Emmprin/CD147/BSG at approximately 35-60 kDa. The expected band size for Emmprin/CD147/BSG is at 42 kDa.
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IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-3).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-3).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-3).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of Emmprin/CD147/BSG using anti-Emmprin/CD147/BSG antibody (A00248-3).
Emmprin/CD147/BSG was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- emmprin/CD147 Antibody (A00248-3). detected in paraffin-embedded section of mouse colon tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
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IF analysis using anti- emmprin/CD147 Antibody (A00248-3). detected in paraffin-embedded section of rat colon tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
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Flow Cytometry analysis of mouse PBMC cells using anti-Emmprin/CD147/BSG antibody (A00248-3).
Overlay histogram showing mouse PBMC cells stained with A00248-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Emmprin/CD147/BSG Antibody (A00248-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Emmprin/CD147/BSG Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

5F7; Basigin; basigin (Ok blood group); BSG; CD147; Collagenase stimulatory factor; EMMPRIN; HAb18G; M6; OK; OK blood group antigen; TCSF

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, IF, FCM, ELISA

基因名称

BSG

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Bsg recombinant protein (Position: S39-R325).

计算分子量

42 kDa

实际分子量

35-60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. BSG has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Uniprot ID

P18572

RRID

AB_3081028

文献引用格式

Emmprin/CD147/BSG Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00248-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

basigin

蛋白名全称

Basigin

实验方案>

实验步骤

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