Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Western blot analysis of anti-Emmprin/CD147/BSG antibody (BM4979). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Emmprin/CD147/BSG antigen affinity purified monoclonal antibody (BM4979) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Emmprin/CD147/BSG at approximately 65 kDa. The expected band size for Emmprin/CD147/BSG is at 42 kDa.
Immunohistochemical analysis of paraffin-embedded human stomach cancer, using CD147 Antibody.
Immunofluorescent analysis of Jurkat cells, using using CD147 Antibody.
Western blot analysis of anti-Emmprin/CD147/BSG antibody (BM4979). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Emmprin/CD147/BSG antigen affinity purified monoclonal antibody (BM4979) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Emmprin/CD147/BSG at approximately 65 kDa. The expected band size for Emmprin/CD147/BSG is at 42 kDa.
Immunohistochemical analysis of paraffin-embedded human stomach cancer, using CD147 Antibody.
Immunofluorescent analysis of Jurkat cells, using using CD147 Antibody.