Anti-NF-κB p65/RELA Antibody

筛选器： All WB ICC/IF IP FCM ELISA

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  Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Raji whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat PC-12 whole cell lysates,
  Lane 6: rat RH-35 whole cell lysates,
  Lane 7: mouse RAW264.7 whole cell lysates,
  Lane 8: mouse Hepa1/6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 60 kDa.

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  Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HAP1 -WT whole cell lysates,
  Lane 2: human HAP1 -RELA KO whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 2: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 60 kDa.

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  IF analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1).
  NF-κB p65/RELA was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NF-κB p65/RELA Antibody (A00284-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  IP analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1) in Hela whole cell lysate.
  Western blot analysis of NF-κB p65/RELA using anti- NF-κB p65/RELA antibody (A00284-1).
  Lane 1: HepG2 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- NF-κB p65/RELA antibody in HepG2 whole cell lysate,
  Lane 3: anti- NF-κB p65/RELA antibody (2μg) + HepG2 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- NF-κB p65/RELA antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 65-70 kDa. The expected band size for NF-κB p65/RELA is at 60 kDa.

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  Flow Cytometry analysis of Hela cells using anti-NF-κB p65/RELA antibody (A00284-1).
  Overlay histogram showing Hela cells stained with A00284-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF-κB p65/RELA Antibody (A00284-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Raji whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat PC-12 whole cell lysates,
  Lane 6: rat RH-35 whole cell lysates,
  Lane 7: mouse RAW264.7 whole cell lysates,
  Lane 8: mouse Hepa1/6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 60 kDa.

• 

  Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HAP1 -WT whole cell lysates,
  Lane 2: human HAP1 -RELA KO whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 2: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 60 kDa.

• 

  IF analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1).
  NF-κB p65/RELA was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NF-κB p65/RELA Antibody (A00284-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  IP analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (A00284-1) in Hela whole cell lysate.
  Western blot analysis of NF-κB p65/RELA using anti- NF-κB p65/RELA antibody (A00284-1).
  Lane 1: HepG2 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- NF-κB p65/RELA antibody in HepG2 whole cell lysate,
  Lane 3: anti- NF-κB p65/RELA antibody (2μg) + HepG2 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- NF-κB p65/RELA antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 65-70 kDa. The expected band size for NF-κB p65/RELA is at 60 kDa.

• 

  Flow Cytometry analysis of Hela cells using anti-NF-κB p65/RELA antibody (A00284-1).
  Overlay histogram showing Hela cells stained with A00284-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF-κB p65/RELA Antibody (A00284-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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