Figure 1. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. -NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
all(10)
Figure 2. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MDA-MB-231 whole cell lysates,Lane 5: human PANC-1 whole cell lysates,Lane 6: human A375 whole cell lysates. -NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
all(10) 
Figure 3. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of human colon cancer tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 4. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of rat small intestine tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 5. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of human mammary cancer tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 6. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of rat kidney tissue . anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(10) 
Figure 7. Immunofluorescence assay analysis of U2OS cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.
all(10) 
Figure 8. Flow cytometry analysis of A431 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(10) 
Figure 9. Flow cytometry analysis of Hela cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(10) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. -NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Figure 2. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MDA-MB-231 whole cell lysates,Lane 5: human PANC-1 whole cell lysates,Lane 6: human A375 whole cell lysates. -NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Figure 3. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of human colon cancer tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of rat small intestine tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of human mammary cancer tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of rat kidney tissue . anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. Immunofluorescence assay analysis of U2OS cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.
Figure 8. Flow cytometry analysis of A431 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 9. Flow cytometry analysis of Hela cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. -NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Figure 2. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MDA-MB-231 whole cell lysates,Lane 5: human PANC-1 whole cell lysates,Lane 6: human A375 whole cell lysates. -NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Figure 3. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of human colon cancer tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of rat small intestine tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of human mammary cancer tissue. anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1).NF-kB p65 was detected in paraffin-embedded section of rat kidney tissue . anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. Immunofluorescence assay analysis of U2OS cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.
Figure 8. Flow cytometry analysis of A431 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 9. Flow cytometry analysis of Hela cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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