Anti-NF-κB p65/RELA Antibody

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3940

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Western blot analysis of NF-κB p65 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HT-1080 cells, using NF-κB p65 Antibody .
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
all(11)
All lanes use the Antibody for 1 hour at room temperature.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NF-κB p65/RELA Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

NF Kappa B; NF κB; NFKB 3; NFKB p65; NFKB3; NF-κB; NF-κB3; p65; RELA; Transcription factor p65

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

RELA

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NF-B p65

计算分子量

60 kDa

实际分子量

65 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.

Uniprot ID

Q04206

文献引用格式

NF-κB p65/RELA Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM3940)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Transcription factor p65

蛋白名全称

Transcription factor p65

实验方案>

实验步骤

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