Anti-NF-κB p65/RELA Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0321

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- NFκBp65(RELA) antibody (PB0321). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates,
Lane 2: mouse Ana-1 whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat PC-12 whole cell lysates.
Use rabbit anti- NFκBp65(RELA) 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NFκBp65(RELA) at approximately 65KD. The expected band size for NFκBp65(RELA) is at 60KD.
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产品名称

Anti-NF-κB p65/RELA Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

NF Kappa B; NF κB; NFKB 3; NFKB p65; NFKB3; NF-κB; NF-κB3; p65; RELA; Transcription factor p65

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

基因名称

RELA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse NF-kB p65 recombinant protein (Position: D291-Q479). Mouse NF-kB p65 shares 77% amino acid (aa) sequence identity with human NF-kB p65.

计算分子量

60 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein encoded by the RELA gene. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.

Uniprot ID

Q04207

文献引用格式

NF-κB p65/RELA Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0321)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

v-rel avian reticuloendotheliosis viral oncogene homolog A

蛋白名全称

Transcription factor p65

实验方案>

实验步骤

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