Anti-Survivin/BIRC5 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00379

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of Survivin using anti- Survivin antibody (A00379). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole cell lysates. -Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.
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Figure 2. IHC analysis of Survivin using anti-Survivin antibody (A00379).
Survivin was detected in paraffin-embedded section of human intestinal cancer tissues. anti-Survivin Antibody (A00379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Survivin using anti-Survivin antibody (A00379).
Survivin was detected in paraffin-embedded section of human intestinal cancer tissues. anti-Survivin Antibody (A00379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Western blot analysis of Survivin using anti-Survivin antibody (A00379).
Lane 1: rat pc-12 whole cell lysate,
Lane 2: human K562 whole cell lysate,
Lane 3: mouse thymus tissue lysate.
- probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.
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产品简介>

产品名称

Anti-Survivin/BIRC5 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; EPR 1; IAP4; SURVIVIN

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB,IHC,ELISA

基因名称

BIRC5

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively.

计算分子量

16 kDa

实际分子量

16-18 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. It is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. And this protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Uniprot ID

O15392

RRID

AB_3081089

文献引用格式

Survivin/BIRC5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00379)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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