Figure 1. Western blot analysis of Anti-BIRC5 antibody (PB0377). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse thymus tissue lysates,
Lane 2: Mouse SP2/0 whole cell lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: Mouse ANA-1 whole cell lysates.
Use rabbit Anti-BIRC5 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BIRC5 at approximately 16KD. The expected band size for BIRC5 is at 16KD.
Figure 2. IHC analysis using anti-BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(4) 
Figure 3. IHC analysis using anti- BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse lymph node tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(4) 
Figure 4. Flow cytometry analysis of NEURO-2A cell(1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(4) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Anti-BIRC5 antibody (PB0377). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse thymus tissue lysates,
Lane 2: Mouse SP2/0 whole cell lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: Mouse ANA-1 whole cell lysates.
Use rabbit Anti-BIRC5 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BIRC5 at approximately 16KD. The expected band size for BIRC5 is at 16KD.
Figure 2. IHC analysis using anti-BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse lymph node tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. Flow cytometry analysis of NEURO-2A cell(1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of Anti-BIRC5 antibody (PB0377). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse thymus tissue lysates,
Lane 2: Mouse SP2/0 whole cell lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: Mouse ANA-1 whole cell lysates.
Use rabbit Anti-BIRC5 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BIRC5 at approximately 16KD. The expected band size for BIRC5 is at 16KD.
Figure 2. IHC analysis using anti-BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse lymph node tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. Flow cytometry analysis of NEURO-2A cell(1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
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