Figure 1. Western blot analysis of anti-DNM1L antibody (A00556-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DNM1L antigen affinity purified polyclonal antibody (A00556-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DNM1L at approximately 82 kDa. The expected band size for DNM1L is at 82 kDa.
Figure 2. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human appendicitis tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human gastric cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 9. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human testicular cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 10. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 11. IF analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in an immunocytochemical section of U2OS cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 12. Flow Cytometry analysis of A549 cells using anti-DNM1L antibody (A00556-2).
Overlay histogram showing A549 cells stained with A00556-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1L Antibody (A00556-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 13. Flow Cytometry analysis of SiHa cells using anti-DNM1L antibody (A00556-2).
Overlay histogram showing SiHa cells stained with A00556-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1L Antibody (A00556-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti-DNM1L antibody (A00556-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DNM1L antigen affinity purified polyclonal antibody (A00556-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DNM1L at approximately 82 kDa. The expected band size for DNM1L is at 82 kDa.
Figure 2. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human appendicitis tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human gastric cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 9. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human testicular cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 10. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 11. IF analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in an immunocytochemical section of U2OS cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 12. Flow Cytometry analysis of A549 cells using anti-DNM1L antibody (A00556-2).
Overlay histogram showing A549 cells stained with A00556-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1L Antibody (A00556-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 13. Flow Cytometry analysis of SiHa cells using anti-DNM1L antibody (A00556-2).
Overlay histogram showing SiHa cells stained with A00556-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1L Antibody (A00556-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of anti-DNM1L antibody (A00556-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DNM1L antigen affinity purified polyclonal antibody (A00556-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DNM1L at approximately 82 kDa. The expected band size for DNM1L is at 82 kDa.
Figure 2. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human appendicitis tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human gastric cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 8. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 9. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human testicular cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 10. IHC analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 11. IF analysis of DNM1L using anti-DNM1L antibody (A00556-2).
DNM1L was detected in an immunocytochemical section of U2OS cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 12. Flow Cytometry analysis of A549 cells using anti-DNM1L antibody (A00556-2).
Overlay histogram showing A549 cells stained with A00556-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1L Antibody (A00556-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 13. Flow Cytometry analysis of SiHa cells using anti-DNM1L antibody (A00556-2).
Overlay histogram showing SiHa cells stained with A00556-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1L Antibody (A00556-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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