Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of anti- DRP1/DNM1L antibody (A00556-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: rat heart tissue lysates.
Use rabbit anti- DRP1/DNM1L 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for DRP1/DNM1L at approximately 75KD. The expected band size for DRP1/DNM1L is at 82KD.
Figure 1. Western blot analysis of anti- DRP1/DNM1L antibody (A00556-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: rat heart tissue lysates.
Use rabbit anti- DRP1/DNM1L 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for DRP1/DNM1L at approximately 75KD. The expected band size for DRP1/DNM1L is at 82KD.