Western blot (WB): | 1:4000 |
Immunohistochemistry (IHC): | 1:150 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNM1L monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNM1L (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNM1L.
Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 6. Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 7. Immunofluorescent staining of HeLa cells using anti-DNM1L mouse monoclonal antibody.
Figure 8. Anti-DNM1L mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNM1L .
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNM1L monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNM1L (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNM1L.
Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 6. Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00556-1)
Figure 7. Immunofluorescent staining of HeLa cells using anti-DNM1L mouse monoclonal antibody.
Figure 8. Anti-DNM1L mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNM1L .