Anti-HDAC3 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of HDAC3 using anti-HDAC3 antibody (A00839). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: human MOLT-4 whole cell lysates,
  Lane 5: human MCF-7 whole cell lysates,
  Lane 6: human K562 whole cell lysates,
  Lane 7: human Caco-2 whole cell lysates,
  Lane 8: rat thymus tissue lysates,
  Lane 9: C6 whole cell lysates,
  Lane 10: ANA-1 whole cell lysates,
  Lane 11: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (A00839) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.

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  Flow Cytometry analysis of MCF-7 cells using anti-HDAC3 antibody (A00839).
  Overlay histogram showing MCF-7 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Raw264.7 cells using anti-HDAC3 antibody (A00839).
  Overlay histogram showing Raw264.7 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of RH-35 cells using anti-HDAC3 antibody (A00839).
  Overlay histogram showing RH-35 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of HDAC3 using anti-HDAC3 antibody (A00839). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human HepG2 whole cell lysates,
  Lane 4: human MOLT-4 whole cell lysates,
  Lane 5: human MCF-7 whole cell lysates,
  Lane 6: human K562 whole cell lysates,
  Lane 7: human Caco-2 whole cell lysates,
  Lane 8: rat thymus tissue lysates,
  Lane 9: C6 whole cell lysates,
  Lane 10: ANA-1 whole cell lysates,
  Lane 11: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (A00839) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.

• 

  Flow Cytometry analysis of MCF-7 cells using anti-HDAC3 antibody (A00839).
  Overlay histogram showing MCF-7 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Raw264.7 cells using anti-HDAC3 antibody (A00839).
  Overlay histogram showing Raw264.7 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of RH-35 cells using anti-HDAC3 antibody (A00839).
  Overlay histogram showing RH-35 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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